After graduating, She appeared on the hit TV show, Cemberimde Gul Oya portraying the character of Zarife. This was her first television series to develop an acting career. Following, she was awarded 'Best Actress' in the Republic of Serbia and Montenegro International TV Festival as she played the best role of Gülizar, in the TV movie Gülizar.
Later in 2005, Buyukustun debuted her role in the television series Ihlamurlar Altinda, in the series she dedicated her life for to character, Filiz. This series became a huge hit all over the world.
Her successful television series are, Gonülcelen, 20 Minutes, Black Money Love, and currently, The Brave and the Beautiful. Besides, she also starred in numerous movies such as Sinav (Omer Faruk Sorak), My Father and My Son, (Cagan Irmak), Ask Your Heart (Yusuf Kurcenli), Memories of the Wind (Ozcan Alper), Tight Dress (Hiner Saleem).
For debuting as Melek, in 2013, Tuba was nominated for Best Actress at the "42 International Emmy Awards," and she was the first actress from Turkey to become the nominee of Emmy award.
Apart from her acting career, Buyukustun is an ambassador of "UNICEF National Goodwill" and also served as an advocate for Children's Rights.
Tuba is the holder of Turkey nationality and belongs to the Turkis ethnicity. Tuba has a long list of a boyfriend. She was in relationships with Tolga Karacelik, Sami Saydan, and Bulent Inal.
Tuba married the Turkish actor, Onur Saylak in Paris, France after dating for a long time. In January 2012, the husband and wife blessed with twin girls Maya and Toprak. After that, the couple continued their relationship for five years and divorced on 5 June 2017.
Buyukustun, who is one of the top actresses of Turkey, has well appreciable roles in several movies and television series. She accumulated a net worth of $10 million from her acting career. She has a beautiful luxurious house that she shared on her Instagram.